VIC激發(fā)波長和發(fā)射波長

VIC激發(fā)波長和發(fā)射波長

VIC是一種熒光染料，初由Thermofisher 開發(fā)。VIC的分子量為1023.38，VIC激發(fā)波長(Ex)為526nm，發(fā)射波長(Em)為543nm。VIC發(fā)射在可見光譜的綠黃色部分。用于在5′端( 可作為探針在各種實(shí)時(shí)熒光 PCR、雜交和基于熒光的遺傳分析中應(yīng)用 )熒光標(biāo)記寡核苷酸。VIC磷酰胺化物是將VIC標(biāo)簽摻入寡核苷酸中方便的構(gòu)建塊。

Figure 1：VIC激發(fā)波長和發(fā)射波長的光譜圖

VIC is a fluorescent dye that was originally developed by Applied Biosystems (now, Thermofisher). VIC emits in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications). VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.

Molecular weight：1023.38

Excitation：526nm

Emission：543nm

Figure 2. Chemical structure for 5-VIC phosphoramidite.

上海峰志儀器有限公司代理銷售美國路陽各種款式熒光蛋白觀測燈，有LUYOR-3280手電筒式熒光蛋白觀察手電筒、LUYOR-3415手持式雙波長熒光蛋白激發(fā)光源、LUYOR-3421臺(tái)式熒光蛋白激發(fā)光源，能夠滿足觀察熒光蛋白在模式動(dòng)物、植物葉片、種子、愈傷等上的表達(dá)。也能激發(fā)各種熒光染料發(fā)光。